Expression construct design including system
choice, mutant choice, generation of expression clones by DNA
manipulation and outsourcing, and screening for the best
expression construct/system.
Focused on provision of cells for protein
purification (membrane proteins, intra and extra-cellular domains,
secreted proteins) for Structural studies.
Scale-up to 25L and optimisation using multiple
expression systems - E.coli, Pichia, Baculovirus
and Mammalian cells - for Protein Structure
determination.
Stable and Transient Mammalian Cell generation
and Expression screening.
Focus on Membrane proteins or membrane
associated targets.
Departmental support to GMM risk assessments and
application for permits to the
regulatory authorities.
Protein Purification.
Established routine Baculovirus generation by
Baculogold and then Bac-to-bac systems, scale
expanded for 6viruses/year to over 100/year
within 5 years before passing on the system.
Stable Mammalian expression vector development
and evaluation, constructed and evaluated several vectors for
stable cell generation involving variation in the Kozak sequence
and IRES sites, which are still in use
in-house.
Generated and evaluated a 2 vector SIN
Lentivirus system.
Part of a team investigating the potential for
Cell Penetrating Peptides in Target
Validation, used flow
cytometry generated data that supported the entry mechanism being
by endocytosis.
Richard Christison
Freelance Protein Expression Scientist
Tel. +46 768 898489